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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
29/01/2024 |
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Data da última atualização: |
29/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; NAIARA ZOCCAL SARAIVA, CNPGL; LETICIA ZOCCOLARO OLIVEIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS. |
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Título: |
Morphology of 16-cell embryo in bovine: Inside cells, compaction, fragmentation and effects of X-sorted semen. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Anatomia, Histologia, Embryologia, v. 53, e13015, 2024. |
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DOI: |
https://doi.org/10.1111/ahe.13015 |
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Idioma: |
Inglês |
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Conteúdo: |
In mouse embryos, inside cells are allocated in 16-cell embryos through a well-orchestrated sequence of events involving compaction and polarization. The emergence of inside cells is of great importance as itl later gives rise to the inner cell mass and epiblast. In this study, we report the sequence of critical events in embryology (compaction, inside cells allocation and fragmentation) in bovine 72 h.p.i. 9-16 cell embryos, while also investigating the effects of X-sorted semen on these events. We found a wide distribution of total cell numbers among embryos, attributed to an asynchronous cleavage pattern and blastomere death. Additionally, 13% of embryos displayed irregular shapes. The establishment of the inside cell compartment increased (p < 0.01) in embryos with more cells. However, only 53.8% of 16-cell embryos presented inside cells. Compaction was present in 32.4% embryos and was positively correlated (p = 0.03, OR 3.02) with the establishment of inside cells, occurring independently of cell number. Fragmentation was present in 36% embryos, being more frequent (p = 0.01) in embryos with lower cell numbers. A possible association between irregular shape and fragmentation was considered (p = 0.06). The use of X-sorted semen had no effect on most evaluated parameters. However, it did have a marked effect on cleavage rate (p < 0.01) and the arrest of 2- and 4- cell embryos. In conclusion, bovine embryos exhibit an asynchronous cleavage pattern, high levels of fragmentation, and demonstrate compaction and inside cell allocation later in development compared to mouse embryos. Semen X-sorting has major effects on cleavage and embryo arrest. Further studies are needed to elucidate the association between irregularly shaped embryos and fragmentation, as well as the effects of sex on inside cell allocation. MenosIn mouse embryos, inside cells are allocated in 16-cell embryos through a well-orchestrated sequence of events involving compaction and polarization. The emergence of inside cells is of great importance as itl later gives rise to the inner cell mass and epiblast. In this study, we report the sequence of critical events in embryology (compaction, inside cells allocation and fragmentation) in bovine 72 h.p.i. 9-16 cell embryos, while also investigating the effects of X-sorted semen on these events. We found a wide distribution of total cell numbers among embryos, attributed to an asynchronous cleavage pattern and blastomere death. Additionally, 13% of embryos displayed irregular shapes. The establishment of the inside cell compartment increased (p < 0.01) in embryos with more cells. However, only 53.8% of 16-cell embryos presented inside cells. Compaction was present in 32.4% embryos and was positively correlated (p = 0.03, OR 3.02) with the establishment of inside cells, occurring independently of cell number. Fragmentation was present in 36% embryos, being more frequent (p = 0.01) in embryos with lower cell numbers. A possible association between irregular shape and fragmentation was considered (p = 0.06). The use of X-sorted semen had no effect on most evaluated parameters. However, it did have a marked effect on cleavage rate (p < 0.01) and the arrest of 2- and 4- cell embryos. In conclusion, bovine embryos exhibit an asynchronous cleavage pattern, high levels of fragmenta... Mostrar Tudo |
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Palavras-Chave: |
Compactação; Fertilização in vitro; Fragmentação; Massa celular interna. |
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Thesagro: |
Bovino; Embrião Animal; Reprodução Animal. |
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Thesaurus Nal: |
Embryo (animal). |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1161417/1/Morphology-of-16-cell-embryo-in-bovine.pdf
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Marc: |
LEADER 02636naa a2200253 a 4500
001 2161417
005 2024-01-29
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1111/ahe.13015$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aMorphology of 16-cell embryo in bovine$bInside cells, compaction, fragmentation and effects of X-sorted semen.$h[electronic resource]
260 $c2024
520 $aIn mouse embryos, inside cells are allocated in 16-cell embryos through a well-orchestrated sequence of events involving compaction and polarization. The emergence of inside cells is of great importance as itl later gives rise to the inner cell mass and epiblast. In this study, we report the sequence of critical events in embryology (compaction, inside cells allocation and fragmentation) in bovine 72 h.p.i. 9-16 cell embryos, while also investigating the effects of X-sorted semen on these events. We found a wide distribution of total cell numbers among embryos, attributed to an asynchronous cleavage pattern and blastomere death. Additionally, 13% of embryos displayed irregular shapes. The establishment of the inside cell compartment increased (p < 0.01) in embryos with more cells. However, only 53.8% of 16-cell embryos presented inside cells. Compaction was present in 32.4% embryos and was positively correlated (p = 0.03, OR 3.02) with the establishment of inside cells, occurring independently of cell number. Fragmentation was present in 36% embryos, being more frequent (p = 0.01) in embryos with lower cell numbers. A possible association between irregular shape and fragmentation was considered (p = 0.06). The use of X-sorted semen had no effect on most evaluated parameters. However, it did have a marked effect on cleavage rate (p < 0.01) and the arrest of 2- and 4- cell embryos. In conclusion, bovine embryos exhibit an asynchronous cleavage pattern, high levels of fragmentation, and demonstrate compaction and inside cell allocation later in development compared to mouse embryos. Semen X-sorting has major effects on cleavage and embryo arrest. Further studies are needed to elucidate the association between irregularly shaped embryos and fragmentation, as well as the effects of sex on inside cell allocation.
650 $aEmbryo (animal)
650 $aBovino
650 $aEmbrião Animal
650 $aReprodução Animal
653 $aCompactação
653 $aFertilização in vitro
653 $aFragmentação
653 $aMassa celular interna
700 1 $aSARAIVA, N. Z.
700 1 $aOLIVEIRA, L. Z.
773 $tAnatomia, Histologia, Embryologia$gv. 53, e13015, 2024.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 182 | |
| 4. |  | OLIVEIRA, C. S.; SARAIVA, N. Z.; OLIVEIRA, L. Z. Morphology of 16-cell embryo in bovine: Inside cells, compaction, fragmentation and effects of X-sorted semen. Anatomia, Histologia, Embryologia, v. 53, e13015, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | CAMARGO, L. S. de A.; OLIVEIRA, C. S.; PFEIFER, L. F. M.; MACHADO, R. Avanços e inovações em biotécnicas da reprodução em bovinos de leite. In: VILELA, D.; FERREIRA, R. de P.; FERNANDES, E. N.; JUNTOLLI, F. V. (Ed.). Pecuária de leite no Brasil: cenários e avanços tecnológicos. Brasília, DF: Embrapa, 2016. p. 325-343.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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| 8. | | CARNEIRO, H.; OLIVEIRA, C. S. DE; LIMA, N. R. DE; ARAÚJO, D. R. C. DE. Análise das representações sociais dos integrantes da cadeia produtiva do leite sobre os impactos sócio-ambientais da atividade leiteira. In: CONGRESSO INTERNACIONAL DO LEITE, 11.; WORKSHOP DE POLÍTICAS PÚBLICAS, 11.; SIMPÓSIO DE SUSTENTABILIDADE DA ATIVIDADE LEITEIRA, 12., 2012, Goiânia. Anais... Juiz de Fora: Embrapa Gado de Leite, 2012.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | OLIVEIRA, C. S. de; DORNIER, M.; REYNES, M.; GOMES, F. dos S.; CABRAL, L. M. C. Concentração de suco de abacaxi pelo acoplamento dos processos de microfiltração, osmose inversa e evaporação osmótica. In: CONGRESO IBERO-AMERICANO EN CIENCIA Y TECNOLOGÍA DE MEMBRANAS, 8., 2012, Salta. Libro de resúmenes. Salta: Universidad Nacional de Salta, 2012. p. 261.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 12. | | GARCIA, J. M.; LIMA, M. R. de; SARAIVA, N. Z.; OLIVEIRA, C. S. Evaluation of L-carnitine supplementation on the production and vitrification of bovine embryos produced in vitro. Animal Reproduction, v. 14, n. 3, p. 882, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 13. | | HORNUNG, P. S.; OLIVEIRA, C. S. de; LAZZAROTTO, M.; LAZZAROTTO, S. R. da S.; SCHNITZLER, E. Investigation of the photo-oxidation of cassava starch granules: thermal, rheological and structural behaviour. Journal of Thermal Analysis and Calorimetry, v. 123, n. 3, p. 2129-2137, Mar. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 182 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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